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Category
Primary Antibodies
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Long description
A bidirectional lipid cholinephosphotransferases capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and the inverse reaction. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. May not function strictly as a SM synthase. Inhibited by bacterial PC-phospholipase C inhibitor D609. Localized to Golgi apparatus; Golgi membrane; multi-pass membrane protein. Possibly present on Brain, heart, kidney, liver, muscle and stomach.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from C57BI/6 mice immunized with a synthetic peptide derived from the human T-catenin protein and SP2/0-Ag14 mouse myeloma cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Sphingomyelin Synthase 1 (Peptide as antigen) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA, Western Blot
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Antibody s suited for
Antibody can be used for Immunofluorescence on acetone-fixed cells, immunocytochemistry (1:25-1:200) on methanol and paraformaldehyde-fixed cells, immunoprecipitation and Western blotting (1:100-1:1000). Immunocytochemistry requires permeabilization with 0.2% Triton X-100 for 2 minutes. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Janssens, B., et al. 'alphaTcatenin: a novel tissue-specific beta-cateninbinding protein mediating strong cell-cell adhesion', J. Cell. Sci. 2001, 114, 3177-88.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method. Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
