BrdU Cell Proliferation Assay Kit

  • Catalog number
    X1327K3
  • Price
    Please ask
  • Size
    5000 Tests
  • Category
    Other Reagents
  • Long description
    see techfile
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with purified monkey recombinant p73a protein (amino acids 380-637) and mouse myeloma NS1 cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide.
  • Clone
    not specified
  • Antigen antibody binding interaction
    BrdU Cell Proliferation Assay Kit
  • Antibody is raised in
    see techfile
  • Antibody s reacts with
    BrdU Incorporated Cells
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    ELISA
  • Antibody s suited for
    This antibody can be used for gel supershift assays on p73 and p73 , immunofluroescence, immunoprecipitation (co precipitates p73 / ; 2 µg/mg of protein lysate) and Western blotting (detects both p73 / ; 2 µg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Storage
    4ºC
  • Relevant references
    1. Dobbelstein M, Roth J: The large T antigen of simian virus 40 binds and inactivates p53 but not p73. J Gen Virol 1998;79:3079-3083._x000B__x000B_2. Higashino F, et al: Adenovirus e4orf6 oncoprotein modulates the function of the p53-related protein, p73. Proc Natl Acad Sci U S A 1998 Dec 22;95(26):15683-15687._x000B__x000B_3. Lo Cunsolo C, et al: Refined chromosomal localization of the putative tumor suppressor gene TP73. Cytogenet Cell Genet 1998;82(3-4):199-201._x000B__x000B_4. Kovalev S, et al: Expression level, allelic origin, and mutation analysis of the p73 gene in neuroblastoma tumors and cell lines. Cell Growth Differ 1998 Nov;9(11):897-903._x000B__x000B_5. Zhu J, et al: The potential tumor suppressor p73 differentially regulates cellular p53 target genes. Cancer Res 1998 Nov 15;58(22):5061-5065._x000B__x000B_6. De Laurenzi V, et al: Two new p73 splice variants, gamma and delta, with different transcriptional activity. J Exp Med 1998 Nov 2;188(9):1763-1768._x000B__x000B_7. Nimura Y, et al: p73, a gene related to p53, is not mutated in esophageal carcinomas. Int J Cancer 1998 Nov 9;78(4):437-440._x000B__x000B_8. Marin MC et. al. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Molecular and Cellular Biology, 1998;18(11):6316-6324.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Test
    nordc supplies other types of Assays as 1.
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
  • Short name
    BrdU Proliferation Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Isotype
    not specified
  • Label
    unlabeled
  • Alternative name
    Bromodeoxyuridine cellular expantion test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
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