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Category
Other Reagents
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Long description
see techfile
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Antibody come from
Synthetic peptide derived from the GPR4 protein.
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Other description
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Chemiluminescent BrdU Cell Proliferation Assay Kit
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Antibody is raised in
see techfile
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Proliferation Assay
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Antibody s suited for
Antibody can be used for Western blotting (1:400 dilution). Tissue lysate should be used at 10-50 µg/lane. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Sin, W.C., et al. 'G protein-coupled receptors GPR4 and TDAG8 are oncogenic and overexpressed in human cancers.' Oncogene 2004_x000B__x000B_2. Yun, M.R., et al. 'The action mode of lysophosphatidylcholine in human monocytes.' J. Pharmacol. Sci. 2004, 94, 45-50_x000B__x000B_3. Bektas, M., et al. 'The G protein-coupled receptor GPR4 suppresses ERK activation in a ligand-independent manner.' Biochemistry 2003, 42, 12181-12191_x000B__x000B_4. Lum, H., et al. 'Inflammatory stress increases receptor for lysophosphatidylcholine in human microvascular endothelial cells.' Am. J. Physiol. Heart Circ. Physiol. 2003, 285, H1786-H1789_x000B__x000B_5. Xu, Y. 'Sphingosylphosphorylcholine and lysophosphatidylcholine: G protein-coupled receptors and receptor-mediated signal transduction.' Biochim Biophys Acta 2002, 1582, 81-88_x000B__x000B_6. Zhu, K., et al. 'Sphingosylphosphorylcholine and lysophosphatidylcholine are ligands for the G protein-coupled receptro GPR4.' J. Biol. Chem. 2001, 276, 41325-41335_x000B__x000B_7. An, S., et al. 'Cloning, sequencing and tissue distribution of two related G protein-coupled receptor candidates expressed prominently in human lung tissue.' FEBS Lett. 1995, 375, 121-124
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Test
nordc supplies other types of Assays as 1.
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Description
For cells, cell lines and tissues in culture till half confluency.
