BrdU Cell Proliferation Assay Kit

• Catalog number
  K306-200
• Price
  Please ask
• Size
  200 assays

• Description
  Non-radioactive Assay, detects as less as 50-100 proliferating cells
• Summary
  • Detection- Absorbance (450 nm) • Applications- Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. - Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
• Detection Method
  Absorbance (450 nm)
• Applications
  • Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. • Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
• Sample Type
  • Adherent and suspension cells
• Features Benefits
  • Convenient & non-radioactive • High-throughput suitable • Highly-sensitive: detect as low as 50-100 proliferating cells. • Suitable for cell proliferation, chemotaxis and cytotoxicity
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Biovision's BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  BrdU   Proliferation   Kit  
• Short name
  BrdU Proliferation Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Bromodeoxyuridine cellular expantion test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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