StemTAG PCR Primer Set for Stem Cell Characterization[StemTAG PCR Primer Set for Stem Cell Characterization]

• Catalog number
  MBS168916
• Price
  Please ask
• Size
  NA

• Products_type
  Kit
• Description
  For cells, cell lines and tissues in culture till half confluency.
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
• Test
  Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

• Gene target
  StemTAG   Primer   Set   for   Stem   Characterization[StemTAG   Primer   Set   for   Stem   Characterization]  
• Short name
  StemTAG PCR Primer Set for Stem Characterization[StemTAG PCR Primer Set for Stem Characterization]
• Technique
  PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  StemTAG PCR test kit Primer Set to measure progenitor cellular Characterization[StemTAG PCR test kit Primer Set to measure progenitor cellular Characterization]
• Alternative technique
  pcr-sequence, dna-amplification
• Tissue
  cell, set, stem

MeSH Data

• Name
  Amplified Fragment Length Polymorphism Analysis
• Concept
  Scope note: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.
• Tree numbers
  • E05.393.290.382
  • E05.393.620.500.324
• Qualifiers
  drug effects, radiation effects, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products