Primer Set for PCR Controls
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Description
Positive controls are often requested for publication of your result. Reference 1 can be used to spike a vast number of experiments
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
Primer Set for PCR Controls
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Technique
Controls, PCR, control, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative name
Primer Set to measure PCR test kit Controls
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Alternative technique
controls, pcr-sequence, dna-amplification
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Tissue
set
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MeSH Data
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Name
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Concept
Scope note:
The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.
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Tree numbers
- E05.393.290.382
- E05.393.620.500.324
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Qualifiers
drug effects, radiation effects, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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