StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

• Catalog number
  CBA-325
• Price
  Please ask
• Size
  96 assays

• Shipping Temp
  Room Temp
• Storage Temp
  4ºC
• Detection Method
  Fluorometric
• Category Product Type
  Kit
• URL link to datasheet
• URL link to SDS
  more (CBA-325) GHS.pdf
• UNSPSC
  41116100
• Product Line
  Stem Cell Research
• Short Description
  Our StemTAG™ 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
• Product feature 1
  Quantify ES cells using fluorescent dye or measure alkaline phosphatase activity (reagents included for both methods)
• Product feature 2
  Stem cells may be recovered for further downstream analysis
• Product feature 3
  Results in 7-10 days, not 3 weeks
• Test
  Cell Biolabs supplies other types of Assays as 1. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
• Description
  For cells, cell lines and tissues in culture till half confluency. Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.

• Gene target
  StemTAG   Stem   Colony   Formation   Recovery   Compatible  
• Short name
  StemTAG Stem Colony Formation Assay ( Recovery Compatible)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  StemTAG progenitor cellular Colony Formation test (cellular Recovery Compatible)
• Alternative technique
  arrays
• Tissue
  cell, stem

MeSH Data

• Name
  Colony-Forming Units Assay
• Concept
  Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
• Tree numbers
  • E01.370.225.500.383
  • E05.200.500.383
  • E05.242.383
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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