Rat Anti-Mouse CD25/IL-2Ra-LE/AF
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Catalog numberMBS673286
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PricePlease ask
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Size5x0.5 mg
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Products_typeAntibody
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Products_short_name[CD25/IL-2R alpha]
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Products_name_syn[Rat Anti-Mouse CD25/IL-2Ra - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD25/IL-2R alpha]
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ClonalityMonoclonal
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Clone[7D4]
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ReactivityMouse
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SpecificityLow affinity alpha chain IL-2 receptor (IL-2R alpha), Mr 55 kDa The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^2 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^1, 3, 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220^low TdT^- sIg^- pre-B II and CD45R/B220^low TdT^- sIgM^+sIgD^- immature B cells, but not on the CD45R/B220^low TdT^+ sIg^- pro-B/pre-B I stage nor on CD45R/B220^high TdT^- sIgM^+sIgD^+ mature B cells.^5, 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.^6, 7 The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (Cat. No. 1600-XX). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.^3, 8, 9
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FormLow Endotoxin, Azide - Free, (LE/AF)
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Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested_applicationFlow Cytometry; Immunoprecipitation
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolFUT3, HPS4, IL36G, IL25, IL22, IL18, IL37, IL6R, IL17C, IL17D
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Short nameAnti-Mouse CD25/IL-2Ra-LE/AF
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Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
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HostRat
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IsotypeRat (Lewis) IgM kappa
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SpeciesMouse, Mouses, Rats
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Alternative nameRat Antibody toMouse CD25/Interleukin-2Ra-LE/AF
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 3 (Lewis blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-04-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHPS4 biogenesis of lysosomal organelles complex 3 subunit 2
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Synonyms gene name
- Hermansky-Pudlak syndrome 4
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Synonyms
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Locus
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Discovery year2001-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Biogenesis of lysosomal organelles complex 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 36 gamma
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Synonyms gene
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Synonyms gene name
- interleukin 1 family, member 9
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 25
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Synonyms gene
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Synonyms gene name
- interleukin 17E
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-26
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Pubmed identfication
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 22
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameinterleukin 18
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Synonyms gene name
- interleukin 18 (interferon-gamma-inducing factor)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-07-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 37
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Synonyms gene
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Synonyms gene name
- interleukin 1 family, member 7 (zeta)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 6 receptor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Interleukin receptors
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 17C
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 17D
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-05-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data