Mouse anti Melanoma Associated Antigen
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Catalog number
MBS570200
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Price
Please ask
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Size
0.1 mg
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Products_type
Antibody
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Products_short_name
[Melanoma Associated Antigen]
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Other_names
[melanoma-associated antigen]
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Clonality
Monoclonal
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Clone
[NKI-C3]
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Reactivity
Human
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Specificity
NKI/C-3 is a MAb which is directed anti a predominantly intracellular protein of Human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized by NKI/C-3 is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three.V-linked glycans were added cotranslationally. Extensive further heterogeneity was geneRated in the Golgi compartment and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with Cathepsin D, which is a marker for lysosomal functions. NKI/C3 originally was described as a marker for melanoma. Recently, it resurfaced as a marker to sepaRate cellular neurothekeoma from other dermal tumors in the differential.
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Form
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Storage_stability
Store at 4 degree C, or in small aliquots at -20 degree C.
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Tested_application
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MAGEA1, CSPG4, MAGED2
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Short name
Mouse anti Melanoma Associated Antigen
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Technique
antigen, Mouse, anti, antibody to, antigenes, mouses
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Host
Mouse
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Isotype
IgG1
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Melanoma Associated protein
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Alternative technique
antigenes, murine, antibodies
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Gene info
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Identity
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Gene
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Long gene name
MAGE family member A1
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Synonyms gene
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Synonyms gene name
- melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
- melanoma antigen family A1
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Synonyms
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Synonyms name
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Locus
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Discovery year
1994-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
MAGE family member D2
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Synonyms gene name
- melanoma antigen family D2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-08-16
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Entrez gene record
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RefSeq identity
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Classification
- MAGE family
- Small nucleolar RNA protein coding host genes
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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