IgY F(ab')2, Chicken (FITC)

• Catalog number
  MBS654805
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Immunoglobulin
• Products_short_name
  [IgY F(ab')2, Chicken]
• Specificity
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgY, anti-Chicken IgY F(ab')2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgY F(c) or anti-Pepsin.
• Purity
  Highly Purified Purified by ion exchange chromatography.
• Form
  Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Conjugated to Fluorescein isothiocyanate (FITC). Free FITC removed by solid phase adsorption.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Properties
  Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
• Group
  f(ab')2s
• Conjugation
  Anti-FITC Antibody

• Short name
  IgY F(ab')2, (FITC)
• Technique
  F(ab')2, FITC, IgY,  F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. Fluorescein, IgYs
• Isotype
  F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgY antibody
• Label
  FITC
• Species
  chicken, Chickens
• Alternative name
  IgY F(Antibody')2, Chicken (fluorecein)
• Alternative technique
  secondairy, fluorescine, bird

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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