Donkey anti Chicken IgY (H+L), conjugated with FITC
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Catalog number
MBS397156
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Price
Please ask
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Size
0.5 mg
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Products_type
Secondary Antibody
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Products_short_name
[IgY]
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Products_gene_name
[IgY]
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Form
Liquid; 0.5 mL, Phosphate Buffered Saline‚ pH 7.2‚ 50% Glycerol with 0.075% Sodium Azide
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Concentration
1.0 mg/mL
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Storage_stability
Store at -20 degree C.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
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Latin name
Equus africanus asinus
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
anti IgY (H+L), conjugated with FITC
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Technique
anti, FITC, IgY, antibody to, antibody Conjugates, Fluorescein, IgYs
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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Isotype
IgY antibody
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Label
FITC
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Species
chicken, Chickens, Donkeys
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Alternative name
Donkey antibody to Chicken IgY (heavy + light chain), coupled including fluorecein
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Alternative technique
antibodies, fluorescine, bird
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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