IgM Murine Negative Control (FITC)
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Catalog number
MBS655500
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Price
Please ask
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Size
100 Tests
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Products_type
Isotype Control
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Products_short_name
[IgM Murine Negative Control]
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Clone
[3H2448]
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Reactivity
Human
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Specificity
Recognizes a rat cell surface marker and cannot be used as a negative control in that species.
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Purity
Highly Purified Purified by gel filtration.
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Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
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Storage_stability
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
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Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
ARAFP2, BRAFP1
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Short name
IgM Murine Negative Control (FITC)
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Technique
Negative, Control, FITC, IgM, controls, Fluorescein, IgMs, negatif
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Isotype
IgM, IgM
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Label
FITC
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Alternative name
Immunoglobulin M Murine Negative reference (fluorecein)
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Alternative technique
controls, fluorescine, immunoglobulin-m
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Tissue
control
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Gene info
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Identity
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Gene
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Long gene name
ARAF pseudogene 2
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma 3611 viral oncogene homolog pseudogene
- v-raf murine sarcoma 3611 viral oncogene homolog 2, pseudogene
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Synonyms
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
BRAF pseudogene 1
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Synonyms gene
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Synonyms gene name
- v-raf murine sarcoma viral oncogene homolog B pseudogene 2
- v-raf murine sarcoma viral oncogene homolog B pseudogene 1
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Synonyms
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Locus
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Discovery year
2003-07-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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