-
Long name
Mouse Monoclonal IgM Isotype Control
-
Antigen
Mouse IgM Isotype Control
-
Species reactivity
Immunoglobulin Isotype Control
-
Negative species reactivity
Human, Other species Not tested (expected negative), Porcine, Mouse, Rat
-
Clonality
Monoclonal Antibody (Mab)
-
Clone
PFR-03
-
Applications
FC, negative control
-
Format conjugation
purified, unconjugated
-
Specificity
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
-
Research area
Immunoglobulin Isotype Controls
-
Concentration
1 mg/ml
-
Storage buffer
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
-
Shipping and storage
Mouse Monoclonal IgM Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
-
Background
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified by precipitation and chromatography
-
-
References
Contact us for the most recent list of references
-
Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
-
Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen, Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
-
Group
isotypes
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
