IgG2a Negative Control Murine (FITC)[IgG2a Negative Control Murine]

Products_type

Isotype Control
Reactivity

Human, Rat
Description

Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

IgG2a Murine [IgG2a Murine]
Short name

IgG2a Negative Control Murine (FITC)[IgG2a Negative Control Murine]
Technique

Negative, Control, FITC, controls, Fluorescein, negatif
Host

Mouse
Isotype

IgG2a
Label

FITC
Alternative name

IgG2a Negative reference Murine (fluorecein)[IgG2a Negative reference Murine]
Alternative technique

controls, fluorescine
Tissue

control

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data