Goat anti Guinea Pig IgM (Fc specific), conjugated with FITC

  • Catalog number
    MBS571876
  • Price
    Please ask
  • Size
    1 mL
  • Products_type
    Secondary Antibody
  • Products_short_name
    [Goat anti Guinea Pig IgM (Fc specific)]
  • Clonality
    Polyclonal
  • Reactivity
    Guinea Pig
  • Specificity
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgM, Fc specific
  • Form
    FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Storage_stability
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
  • Tested_application
    ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC)(Frozen), (In)direct Immunofluorescence (IF)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus
  • About
    Pigs and the smaller guinea pigs are frequent used as models for humans.
  • Gene target
  • Short name
    Goat anti IgM (Fc specific), conjugated with FITC
  • Technique
    anti, FITC, Goat, IgM, FC, antibody to, antibody Conjugates, Fluorescein, goats, IgMs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgM, IgM
  • Label
    FITC
  • Species
    Guinea Pig, Goats, Guinea Pigs, Pigs and Guinea pigs
  • Alternative name
    caprine antibody to Guinea swine Immunoglobulin M (fragment c specific), coupled including fluorecein
  • Alternative technique
    antibodies, caprine, fluorescine, immunoglobulin-m
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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