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Alternative name1
Anti-Goat anti Guinea Pig IgM (Fc specific), conjugated with Horseradish peroxidase
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Alternative name2
Caprine anti Guinea Pig IgM (Fc specific), conjugated with Horseradish peroxidase
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Alternative name3
Caprinae anti Guinea Pig IgM (Fc specific), conjugated with Horseradish peroxidase
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Alternative name4
Goat anti Guinea Pig IgM (Fc specific)
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Alternative name5
N/A
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Secondary Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
N/A
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Clone
Polyclonal antibody
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Host organism
Goat
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Species reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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Specificity and cross reactivity
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
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Purification method
Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
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Form Appearance
Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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Concentration
8mg/ml
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Storage and shipping
Storage I shelf life: Lyophilized at +Store productone at +4 degrees Celsius. = at least 10 years reconstituted at or below Store productone at -20 degrees Celsius. = 3-5 years reconstituted at +Store productone at +4 degrees Celsius.= 7 days
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Tested for
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
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Latin name
Capra aegagrus hircus
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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About
Pigs and the smaller guinea pigs are frequent used as models for humans.
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Gene target
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Short name
Goat anti IgM (Fc specific), conjugated with Horseradish
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Technique
anti, Goat, IgM, FC, antibody to, antibody Conjugates, goats, IgMs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgM, IgM
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Label
horseradish
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Species
Guinea Pig, Goats, Guinea Pigs, Pigs and Guinea pigs
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Alternative name
caprine antibody to Guinea swine Immunoglobulin M (fragment c specific), coupled including Horseradish peroxidase
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Alternative technique
antibodies, caprine, immunoglobulin-m
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