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Products_type
Secondary Antibody
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Products_short_name
[Goat anti Guinea Pig IgM (Fc specific)]
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Clonality
Polyclonal
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Reactivity
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Specificity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgM, Fc specific
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Form
HRP; Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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Storage_stability
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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Tested_application
ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC-P), Dot blot, Immunoblotting.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
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Latin name
Capra aegagrus hircus
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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About
Pigs and the smaller guinea pigs are frequent used as models for humans.
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Gene target
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Short name
Goat anti IgM (Fc specific), conjugated with Horseradish
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Technique
anti, Goat, IgM, FC, antibody to, antibody Conjugates, goats, IgMs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgM, IgM
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Label
horseradish
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Species
Guinea Pig, Goats, Guinea Pigs, Pigs and Guinea pigs
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Alternative name
caprine antibody to Guinea swine Immunoglobulin M (fragment c specific), coupled including Horseradish peroxidase
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Alternative technique
antibodies, caprine, immunoglobulin-m
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