CD134 Ligand (CD134L, OX40L, CD252) (FITC)
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Catalog number
MBS600114
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Price
Please ask
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Size
120 Tests
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Products_type
Antibody
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Products_short_name
[CD134 Ligand]
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Products_name_syn
[Anti -CD134 Ligand (CD134L, OX40L, CD252) (FITC)]
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Other_names
[CD134(OX40)-ligand; tumor necrosis factor ligand superfamily member 4; CD134(OX40)-ligand]
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Other_gene_names
[TNFSF4]
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Clonality
Monoclonal
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Clone
[7H146]
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Reactivity
Human
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Specificity
Binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.
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Purity
Affinity Purified Purified by Protein A affinity chromatography.
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Form
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with FITC. Molar Ratio: 8.1 FITC /IgG.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Gene target
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Gene symbol
TNFRSF4, TNFSF4
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Short name
CD134 Ligand (CD134L, OX40L, CD252) (FITC)
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Technique
FITC, Fluorescein
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Alternative name
CD134 Ligand (CD134L, OX40L, CD252) (fluorecein)
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Alternative technique
fluorescine
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Gene info
Gene info
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Identity
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Gene
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Long gene name
TNF superfamily member 4
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Synonyms gene
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Synonyms gene name
- tax-transcriptionally activated glycoprotein 1, 34kD
- tumor necrosis factor (ligand) superfamily, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year
1993-12-15
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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