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Synonyms
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1
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Alternative_names
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1
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Description
A costimulatory molecule implicated in long-term T-cell immunity, also cts as a receptor for human herpesvirus 6B/HHV-6B
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
> 90%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Mouse OX40, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse OX40 Ligand, His Tag with a linear range of 0.5-16 ng/mL
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Binding Capacity
Immobilized Mouse OX40, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse OX40 Ligand, His Tag with a linear range of 0.5-16 ng/mL
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Molecular Weight
18.3 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TNFRSF4, TNFSF4
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Short name
CellExp™ OX40 Ligand / TNFSF4, Mouse Recombinant
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
mouse
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Species
Humans, Mouses
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Alternative name
H. sapiens CellExp™ OX40 Ligand / tumor necrosis factor (ligand) superfamily, member 4, Mouse Rec.
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Alternative technique
rec, murine
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Alternative to gene target
tumor necrosis factor (ligand) superfamily, member 4, CD134L and CD252 and GP34 and OX-40L and OX4OL and TXGP1, TNFSF4 and IDBG-104864 and ENSG00000117586 and 7292, tumor necrosis factor receptor superfamily binding, Extracellular, Tnfsf4 and IDBG-201525 and ENSMUSG00000026700 and 22164, TNFSF4 and IDBG-632319 and ENSBTAG00000002894 and 539800
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