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Also known as
PAG1 / PAG
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Other names
phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; transmembrane phosphoprotein Cbp; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; phosphoprotein associated with glycosphingolipid microdomains 1; phosphoprotein membrane anchor with glycosphingolipid microdomains 1; Csk-binding protein; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
PAG1
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Gene name synonims
PAG
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Other gene names
PAG1; PAG1; CBP; PAG; CBP; PAG
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
MEM-255
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein A Purified
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Form Appearance
PBS, 15 mM sodium azide, approx., pH 7.4.
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
PAG1
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Short name
Mouse Monoclonal [clone MEM-255] (IgG2a) PAG1 / PAG Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG2a
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone MEM-255] (IgG2a) to Human PAG1 / PAG
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Alternative technique
antibodies, murine
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Alternative to gene target
phosphoprotein associated with glycosphin this GO lipid microdomains 1, PAG1 and IDBG-26542 and ENSG00000076641 and 55824, SH2 domain binding, Plasma membranes, Pag1 and IDBG-131044 and ENSMUSG00000027508 and 94212, PAG1 and IDBG-644850 and ENSBTAG00000014026 and 614460
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Clone name
MEM-255
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