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Also known as
Mast Cell Chymase
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Other names
mast cell chymase; Chymase; chymase; alpha-chymase; chymase, heart; OTTHUMP00000178751; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Gene name synonims
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Other gene names
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
8H183
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human Chymase at ~30kD. No cross reactivity with rmMCPT-1, rmMCPT-6, rmMCPT-7 or rmMCPT-11. Species sequence homology: canine (82%), mouse chymase1(75%); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
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Concentration
N/A
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Storage and shipping
Lyophilized powder may be stored the antibody should be stored at -20 degrees Celsius.. Stable for 12 months the antibody should be stored at -20 degrees Celsius.. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
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Description
For cells, cell lines and tissues in culture till half confluency.