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Also known as
Mast Cell Chymase
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Other names
mast cell chymase; Chymase; chymase; alpha-chymase; chymase, heart; OTTHUMP00000178751; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Gene name synonims
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Other gene names
CMA1; CMA1; CYH; MCT1; chymase; MGC119890; MGC119891; CYH; CYM
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
3F258
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2414-05X.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 12 months atStore the antibody at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunohistochemistry (IHC)
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Description
For cells, cell lines and tissues in culture till half confluency.
