Integrin alphaV, beta3 (FITC) Antibody
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Catalog numberGEN600100
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Price:
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Size100ug
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Also known asIntegrin alphaV, beta3
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Other namesintegrin beta-3; N/A; integrin beta-3; beta3 integrin; integrin beta 3; glycoprotein GPIIIa; N/A; N/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameITGB3; N/A; CD61; N/A
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Gene name synonimsITGB3; N/A; CD61; N/A
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Other gene namesITGB3; N/A; CD61; N/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1
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Clone2Q880
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Host organismMouse (Mus musculus)
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Source organismHuman (Homo sapiens)
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Species reactivityAvian, Bovine, Canine, Chicken, Human (Homo sapiens), Monkey, Porcine, Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityRecognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: bovine, chick, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodAffinity Purified by Protein A affinity chromatography.
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Form AppearanceSupplied as a liquid in PBS pH 7.6, 0.1% sodium azide, 15mg/ml BSA, before the addition of glycerol to 40%. Labeled with FITC.
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ConcentrationN/A
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Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. Long-term storage should be done after aliquoting the antibody and freezing the aliqotes at -20 degrees Celsius. Under these conditions the aliquots will be stable for at least one year if kept at -20 °C. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applicationsImmunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Short nameIntegrin alphaV, beta3 (FITC) Antibody
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameAnti-Integrin alphaV, beta3 (FITC)
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Alternative techniqueantibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data