Iscove’s Modified Dulbecco’s Medium (IMDM) w/ L-Glutamine and 25mM HEPES Buffer w/o Sodium bicarbonate and Phenol red

• Catalog number
  AT188-1LX26
• Price
  Please ask
• Size
  26X1L

• Description
  Iscove's modified Dulbecco's medium made by himedia laboratory is enriched with modification of Dulbecco's Modified Eagle's Medium. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. IMDM was the first means using a HEPES buffer.
• Similar products
• Shelf Life
  Use before the expiry date
• Storage conditions
  stored at 2-8°C
• Appearance
  Off-white to Creamish white, homogenous powder
• Solubility
  Clear solution at 17.7 gms/L
• Use
  used in laboratory chemicals, Manufacture of substances
• Technical data
• Data sheet
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Group
  media
• Conjugation
  red dye

• Gene target
  Iscove’s   Modified   Dulbecco’s   IMDM   L-Glutamine   25mM   w/o   Sodium   bicarbonate   Phenol   red  
• Short name
  Iscove’s Modified Dulbecco’s Medium (IMDM) w/ L-Glutamine 25mM w/o Sodium bicarbonate Phenol red
• Technique
  Medium, cell culture Media
• Label
  Red
• Alternative name
  Iscove’s Modified Dulbecco’s Medium (IMDM) w/ L-Glutamine and 25mM HEPES solution w/o Sodium bicarbonate and Phenol red
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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