Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, 25mM HEPES Buffer, L-Glutamine, Sodium pyruvate w/o Sodium bicarbonate and Phenol red

• Catalog number
  AT187-10X1LX3
• Price
  Please ask
• Size
  3X10X1L

• Description
  Dulbecco's Modified Eagle Medium made b himadia laboratory is a modification of Basal Medium Eagle (BME) that contains four times the concentration of amino acids and vitamins. . produce at the beginning of a crop and increases the capacity buffer of the medium.
• Similar products
• Shelf Life
  Use before the expiry date
• Storage conditions
  stored at 2-8°C
• Appearance
  Off-white to Creamish white, homogenous powder
• Solubility
  Clear solution at 19.5 gms/L.
• Use
  used in laboratory chemicals, Manufacture of substances
• Technical data
• Data sheet
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. The Liter or liter is a metric system unit of volume equal to 1 cubic decimeter (dm3), 1,000 cubic centimeters (cm3) or 1/1,000 cubic meter. A cubic decimeter (or liter) occupies a volume of 10×10×10 centimeters (see figure) and is thus equal to one-thousandth of a cubic meter. Water baths of various volumes are available for lab research. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Group
  media
• Conjugation
  red dye

• Gene target
  Dulbecco’s   Modified   Eagle   DMEM   glucose   5gms   Glucose   per   25mM   L-Glutamine   Sodium   pyruvate   w/o   Sodium   bicarbonate   Phenol   red  
• Short name
  Dulbecco’s Modified Eagle Medium (DMEM), glucose w/ 4 5gms Glucose per , 25mM , L-Glutamine, Sodium pyruvate w/o Sodium bicarbonate Phenol red
• Technique
  Medium, cell culture Media
• Label
  Red
• Alternative name
  Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, 25mM HEPES solution, L-Glutamine, Sodium pyruvate w/o Sodium bicarbonate and Phenol red
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  In Vitro Techniques
• Concept
  Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
• Tree numbers
  • E05.481
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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