Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gm Glucose per liter, L-Glutamine, Sodium pyruvate and 25mM HEPES buffer w/o Phenol red and Sodium bicarbonate

• Catalog number
  AT241-1LX26
• Price
  Please ask
• Size
  26X1L

• Description
  Dulbecco's Modified Eagle Medium (DMEM), High Glucose, w/ 4.5gms GLucose per litre, L-GLutamine, Sodium pyruvate and 25mM HEPES buffer, w/o Phenol red and Sodium bicarbonate.Produced by HiMedia Laboratories is a Off-white to Creamish white, homogenousused in laboratory chemicals, Manufacture of substances
• Similar products
• Shelf Life
  Use before the expiry date
• Storage conditions
  store between 2-8°C
• Appearance
  Off-white to Creamish white, homogenous
• Solubility
  Soluble in water
• Use
  used in laboratory chemicals, Manufacture of substances
• Technical data
  Please contact us to request the technical data
• Data sheet
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Group
  media
• Conjugation
  red dye

• Gene target
  Dulbecco’s   Modified   Eagle   DMEM   glucose   5gm   Glucose   per   L-Glutamine   Sodium   pyruvate   25mM   w/o   Phenol   red   Sodium   bicarbonate  
• Short name
  Dulbecco’s Modified Eagle Medium (DMEM), glucose w/ 4 5gm Glucose per , L-Glutamine, Sodium pyruvate 25mM w/o Phenol red Sodium bicarbonate
• Technique
  Medium, cell culture Media
• Label
  Red
• Alternative name
  Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gm Glucose per liter, L-Glutamine, Sodium pyruvate and 25mM HEPES solution w/o Phenol red and Sodium bicarbonate
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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