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Description
Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/ 4.5gms Glucose per liter and 25mM HEPES buffer w/o L-Glutamine, Sodium bicarbonate and Sodium pyruvate, is a white to light pink, homogenous powder. It was originally used to culture embryonic mouse cells.
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Shelf life
Use before expiry date given on the product label.
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Storage
Store at 2-8°C away from bright light
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Appearance
White to light pink, homogenous powder
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PH without Sodium Bicarbonate
NA
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PH with Sodium Bicarbonate
NA
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Osmolality without Sodium Bicarbonate
NA
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Osmolality with Sodium Bicarbonate
NA
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Cultural Response
NA
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Endotoxin Content
NA
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Solubility
NA
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Technical Data
Contact us to request a Technical Data
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Ordering
To order Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/ 4.5gms Glucose per liter and 25mM HEPES buffer w/o L-Glutamine, Sodium bicarbonate and Sodium pyruvate, please use the Cat. Nr.AT067A-10X5L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/ 4.5gms Glucose per liter and 25mM HEPES buffer w/o L-Glutamine, Sodium bicarbonate and Sodium pyruvate
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Group
media
