Goat IgG FITC
-
Catalog number65C-CH0502
-
PricePlease ask
-
Size1 mg
-
-
CategoryImmunoglobulins
-
Antibody SubtypeConjugated Immunoglobulins
-
Area of researchImmunology
-
ReactivityGoat
-
Conjugate TagFITC
-
Method of PurificationGoat IgG (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
-
Form BufferLyophilized from 0.02M K3PO4, pH 7.2, 0.15M NaCl, 10 mg/ml BSA and 0.01% NaN3. I mMunoglobulin and Protease free.
-
StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditionsBlue Ice
-
Tested forIF
-
Usage RecommendationsOptimal conditions to be determined by end user
-
PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
Latin nameCapra aegagrus hircus
-
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Short nameGoat IgG FITC
-
TechniqueFITC, Goat, IgG, Fluorescein, goats, IgGs
-
HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
IsotypeIgG, IgG
-
LabelFITC
-
SpeciesGoats
-
Alternative namePurified Goat IgG FITC conjugate
-
Alternative techniquecaprine, fluorescine, immunoglobulins
-
MeSH Data
-
Name
-
ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data