Goat anti Swine IgM FITC
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Catalog number43C-CB1416
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PricePlease ask
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Size1 mg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Raised inGoat
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SpecificityPig
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Cross ReactivityAntiserum was solid phase adsorbed to ensure class specificity
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IsotypesIgG
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Conjugate TagFITC
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Method of PurificationGoat anti Swine IgM (FITC) was purified by affinity chromatography.
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Form BufferPBS with 0.2% BSA and 0.09% NaN3.
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StorageStore at 4 deg C.
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Shipping conditionsBlue Ice
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Tested forICC; IHC; IF
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Usage RecommendationsICC, IHC and IF: 1:50 - 1:500
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus
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Gene target
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Short nameGoat anti Swine IgM FITC
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Techniqueanti, FITC, Goat, IgM, antibody to, Fluorescein, goats, IgMs
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgM, IgM
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LabelFITC
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SpeciesGoats
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Alternative nameGoat anti Swine IgM secondary antibody (FITC)
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Alternative techniqueantibodies, caprine, fluorescine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data