Goat anti Mouse IgG + IgM FITC

  • Catalog number
    43R-1388
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Mouse IgG + IgM
  • Raised in
    Goat
  • Specificity
    This antibody reacts with heavy (gamma) chains on mouse IgG and heavy (mu) chains on mouse IgM and light chains on all mouse immunoglobulins.
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin mouse serum proteins.
  • Conjugate Tag
    FITC
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Mouse IgG + IgM
  • Source
    Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
  • Storage
    Store at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC; IF; IHC
  • Usage Recommendations
    The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
  • Conjugation
    Anti-FITC Antibody
  • Latin name
    Capra aegagrus hircus, Mus musculus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Gene target
    -
  • Short name
    Goat anti Mouse IgG + IgM FITC
  • Technique
    anti mouse, Mouse, anti, FITC, Goat, IgG, IgM, antibody to, anti mouse antibodies, Fluorescein, goats, IgGs, IgMs, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgG, IgM, IgG, IgM
  • Label
    FITC
  • Species
    Mouse, Goats, Mouses
  • Alternative name
    Goat anti Mouse IgG + IgM secondary antibody (FITC)
  • Alternative technique
    murine, antibodies, caprine, fluorescine, immunoglobulins, immunoglobulin-m
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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