Goat anti Monkey IgM FITC
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Catalog number43R-IG082ft
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Price:
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Size1 mg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenGoat anti-monkey IgM (FITC) was raised in goat using monkey IgM ..
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Raised inGoat
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SpecificityMonkey IgM
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Cross ReactivityShould be determined by the end user
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Conjugate TagFITC
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Method of PurificationGoat anti Monkey IgM (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography.
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Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; FC; IF
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Usage RecommendationsELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus
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Short nameGoat anti IgM FITC
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Techniqueanti, FITC, Goat, IgM, antibody to, Fluorescein, goats, IgMs
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgM, IgM
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LabelFITC
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SpeciesMonkey, Goats, Monkeys
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Alternative nameGoat anti Monkey IgM secondary antibody (FITC)
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Alternative techniqueantibodies, caprine, fluorescine, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data