FLAG antibody
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Catalog number10R-2928
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PricePlease ask
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Size100 µg
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CategoryPrimary Antibody
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Antibody SubtypeMonoclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenFLAG antibodies were raised in mouse using DYKDDDDK (FLAG) synthetic peptide conjugated to KLH ..
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Raised inMouse
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SpecificityFLAG tag
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IsotypesIgG2b
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CloneFG4R
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Method of PurificationFLAG antibody was purified by Protein A affinity chromatography.
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Concentration1 mg/ml
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Form BufferProvided as a liquid in 10 mM PBS, pH 7.2, with 0.05% NaN3
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StorageStore at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forDot Blot; ELISA; IP; WB
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Usage RecommendationsWB: 1:1000-3000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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French translationanticorps
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Short nameantibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelFlag
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Alternative nameMouse monoclonal FLAG antibody
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data