FLAG Octapeptide (FLAG), OVA Conjugated
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Catalog number
RPU50033
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Price
Please ask
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Size
100 μg
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Verified reactivity
Multispecies (General)
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Protein number
Please refer to Uniprot
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Gene number
Please refer to GenBank
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Other name
FLAG epitope; FLAG-Tag
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Protein origin
Peptide Synthesis
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Protein region
Please contact us.
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Protein sequence
Please contact us.
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Information about sequence
Please contact us.
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Protein purity
≥ 90%
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Verified applications
Immunogen; SDS-PAGE; WB
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Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
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Use before
1 year
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Shipping requirements
Blue ice
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Estimated production time
7-11 business days
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Notes
For research use only. Not for diagnostic procedures.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Gene
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
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Gene target
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Short name
Octapeptide ( ), OVA Conjugated
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Technique
antibody Conjugates
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Label
no tag
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Alternative name
FLAG Octapeptide (FLAG), OVA coupled
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