FLAG antibody

• Catalog number
  10R-2139
• Price
  Please ask
• Size
  100 µl

• Category
  Primary Antibody
• Antibody Subtype
  Monoclonal Antibodies
• Area of research
  Miscellaneous
• Type of Immunogen
  FLAG antibodies were raised in Mouse using synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH ..
• Raised in
  Mouse
• Isotypes
  IgG2b
• Clone
  2F11G1
• Concentration
  1.0 mg/ml
• Form Buffer
  Provided as a liquid in ascitic fluid containing 0.03% NaN3.
• Storage
  Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; WB
• Usage Recommendations
  ELISA: 1:10,000, WB: 1:500-1:2000
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• French translation
  anticorps

• Short name
  antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Flag
• Alternative name
  Mouse monoclonal FLAG antibody
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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