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Long name
Mouse Monoclonal Antibody against Human Blood Group Lewis b, Clone: 2-25LE
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Antigen
Blood Group Lewis b
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Species reactivity
Other not tested, Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
2-25LE
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Applications
IHC(P), WB, ELISA
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Immunogen
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
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Format conjugation
purified; unconjugated
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Specificity
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
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Research area
Blood Group Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline with 15 mM sodium azide, pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human Blood Group Lewis b, Clone: 2-25LE is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6. _x000D_ , *First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Exbio supplies this blood related product in 1.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps