CD15 / Lewis x Blood Group antigen monoclonal antibody
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Catalog number1P-213-T100
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PricePlease ask
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Size100 assays
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Long nameMouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158
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AntigenCD15
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Species reactivityHuman (Homo sapiens)
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Negative species reactivityPorcine, Bovine, Sheep
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ClonalityMonoclonal Antibody (Mab)
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CloneMEM-158
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ApplicationsFC, IP, MC
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ImmunogenHuman granulocytes
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Format conjugationPE
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SpecificityThe antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells._x000D_ HLDA VI; WS Code AS A053
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Research areaBlood Group Antigens (Human), CD and Related Antigens (Human)
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Storage bufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
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Shipping and storageMouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by affinity chromatography
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References*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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DescriptionAntigens are peptides or recombinant or native dependent on the production method. Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Exbio supplies this blood related product in 1.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolFUT4
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Short nameAnti-CD15 / Lewis x Blood Group antigen Mab
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TechniqueAntibody, antigen, blood, antibodies against human proteins, antibodies for, antigenes, blood samples, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgM
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Alternative nameMouse Monoclonal to CD15 / Lewis x Blood Group antigen
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Alternative techniqueantibodies, antigenes, sera
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Tissueblood
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Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 4
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Synonyms gene
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Synonyms gene name
- ELAM ligand fucosyltransferase
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Synonyms
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Synonyms name
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Locus
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Discovery year1992-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Fucosyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data