CD15 / Lewis x Blood Group antigen monoclonal antibody

• Catalog number
  11-213-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Mouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158
• Antigen
  CD15
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  Porcine, Bovine, Sheep
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  MEM-158
• Applications
  FC, IP, MC
• Immunogen
  Human granulocytes
• Format conjugation
  purified; unconjugated
• Specificity
  The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells._x000D_ HLDA VI; WS Code AS A053
• Research area
  Blood Group Antigens (Human), CD and Related Antigens (Human)
• Concentration
  1 mg/ml
• Storage buffer
  Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by precipitation and chromatography
• Datasheet
• References
  *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Description
  Antigens are peptides or recombinant or native dependent on the production method. Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Exbio supplies this blood related product in 1.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD15   Lewis   Group   monoclonal  
• Gene symbol
  FUT4
• Short name
  Anti-CD15 / Lewis x Blood Group antigen Mab
• Technique
  Antibody, antigen, blood, antibodies against human proteins, antibodies for, antigenes, blood samples, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgM
• Alternative name
  Mouse Monoclonal to CD15 / Lewis x Blood Group antigen
• Alternative technique
  antibodies, antigenes, sera
• Tissue
  blood

Gene info

• Identity
  HGNC:4015
• Gene
  FUT4
• Long gene name
  fucosyltransferase 4
• Synonyms gene
  • CD15
  • FCT3A
  • ELFT
• Synonyms gene name
  • ELAM ligand fucosyltransferase
• Synonyms
  • FUC-TIV
• Synonyms name
  • galactoside 3-L-fucosyltransferase
  • alpha (1,3) fucosyltransferase, myeloid-specific
• Locus
  11q21
• Discovery year
  1992-04-06
• Entrez gene record
  2526
• Pubmed identfication
  • 1702034
• RefSeq identity
  • NM_002033
• Classification
  • CD molecules
  • Fucosyltransferases
• VEGA ID
  OTTHUMG00000167795

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products