CD45 FITC, Single-Color Reagent
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Catalog number0452
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Price:
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Size100Tests
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Host SourceMouse
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Clone NameBHPT-1
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Product CategoryFlow Cytometry
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Species ReactivityHuman
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ApplicationFlow Cytometry
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ImmunogenDerived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
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Purification MethodProtein A/G Chromatography
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Shipping ConditionsRoom Temperature
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Storage conditionsCD45 FITC, Single-Color Reagent should be stored at 4-8ÂșC. DO NOT FREEZE
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PropertiesThis Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Gene symbolPTPRCAP, PTPRC
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Short nameCD45 FITC, Single-Color Reagent
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TechniqueFITC, Fluorescein
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IsotypeIgG1
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LabelFITC
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Alternative nameCD45 fluorecein, Single-Color Reagent
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Alternative techniquefluorescine
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data