CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: Alexa Fluor 647 conj. Clone: OX-6; antibody-Rat, Triple Color Reagent Cocktail; flow

• Catalog number
  YSRTTC020
• Price
  Please ask
• Size
  vial

• Alternative name 1
  CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: Alexa Fluor 647 conj. Clone: OX-6; anti-Rat, Triple Color Reagent Cocktail; flow
• Alternative name 2
  CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: Alexa Fluor 647 conj. Clone: OX-6; antibody-Rat, Triple Color Reagent Cocktail; flow
• Alternative name 3
  CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: Alexa Fluor 647 conj. Clone: OX-6; antibody-Rattus norvegicustus norvegicus, Triple Color Reagent Cocktail; flow
• Alternative name 4
  anti-CD3: Clone: 1F4/ CD25: Clone: OX-39/ RT1B: Alexa Fluor 647 Clone: OX-6 Triple Color Reagent Cocktail flow
• Alternative name 5
  CD3: Clone: 1F4/ CD25: Clone: OX-39/ RT1B: Alexa Fluor 647 Clone: OX-6 Triple Color Reagent Cocktail flow
• Clonality
  Monoclonal Antibody
• Description
  This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
• Storage and handling
  Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
• Advisory
  To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. accurate-monoclonals made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: conj. Clone: OX-6; antibody- , Triple Color Reagent Cocktail; exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 647, Anti-FITC Antibody
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• French translation
  anticorps

• Gene target
  CD3   conj   1F4   CD25   RPE   conj   OX-39   RT1B   conj   OX-6   Triple   Color   Reagent   Cocktail  
• Gene symbol
  HCRT, CD200, TNFSF4, RPE, SNORD115-39, IGKV1-39, ISG20, IGKV1D-39, IGHV4-39, ZNF140
• Short name
  CD3: FITC conj. Clone: 1F4/ CD25: RPE conj. Clone: OX-39/ RT1B: conj. Clone: OX-6; antibody- , Triple Color Reagent Cocktail;
• Technique
  Antibody, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein
• Host
  Rat
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  CD3: fluorecein conj. clonality: 1F4/ CD25: RPE conj. clonality: OX-39/ RT1B: Alexa Fluor 647 conj. clonality: OX-6; (antibody to-)-Rat, Triple Color Reagent Cocktail; flow
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:4847
• Gene
  HCRT
• Long gene name
  hypocretin neuropeptide precursor
• Synonyms gene name
  • hypocretin (orexin) neuropeptide precursor
• Synonyms
  • PPOX
  • OX
• Synonyms name
  • prepro-orexin
  • orexin
• GenBank acession
  • AF041240
• Locus
  17q21.2
• Discovery year
  1998-04-21
• Entrez gene record
  3060
• Pubmed identfication
  • 9419374
  • 9491897
• RefSeq identity
  • NM_001524
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000180107

Gene info

• Identity
  HGNC:7203
• Gene
  CD200
• Long gene name
  CD200 molecule
• Synonyms gene
  • MOX1
  • MOX2
• Synonyms gene name
  • antigen identified by monoclonal antibody MRC OX-2
  • CD200 antigen
• Synonyms
  • MRC
  • OX-2
• Locus
  3q13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4345
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000159248

Gene info

• Identity
  HGNC:11934
• Gene
  TNFSF4
• Long gene name
  TNF superfamily member 4
• Synonyms gene
  • TXGP1
• Synonyms gene name
  • tax-transcriptionally activated glycoprotein 1, 34kD
  • tumor necrosis factor (ligand) superfamily, member 4
• Synonyms
  • OX-40L
  • gp34
  • CD252
• GenBank acession
  • D90224
• Locus
  1q25.1
• Discovery year
  1993-12-15
• Entrez gene record
  7292
• Pubmed identfication
  • 1996093
  • 8076595
• Classification
  • Tumor necrosis factor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000034838

Gene info

• Identity
  HGNC:10293
• Gene
  RPE
• Long gene name
  ribulose-5-phosphate-3-epimerase
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  6120
• RefSeq identity
  • NM_006916
• VEGA ID
  OTTHUMG00000154690

Gene info

• Identity
  HGNC:33058
• Gene
  SNORD115-39
• Long gene name
  small nucleolar RNA, C/D box 115-39
• Synonyms
  • HBII-52-39
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033813
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003354.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5740
• Gene
  IGKV1-39
• Long gene name
  immunoglobulin kappa variable 1-39
• Synonyms gene name
  • immunoglobulin kappa variable 1-39 (gene/pseudogene)
• GenBank acession
  • X59315
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28930
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151678

Gene info

• Identity
  HGNC:6130
• Gene
  ISG20
• Long gene name
  interferon stimulated exonuclease gene 20
• Synonyms gene name
  • interferon stimulated exonuclease gene 20kDa
• Synonyms
  • HEM45
  • CD25
• GenBank acession
  • X89773
• Locus
  15q26.1
• Discovery year
  1998-01-30
• Entrez gene record
  3669
• Pubmed identfication
  • 9235947
  • 9605874
• RefSeq identity
  • NM_002201
• Classification
  • Exonucleases
• VEGA ID
  OTTHUMG00000148679

Gene info

• Identity
  HGNC:5756
• Gene
  IGKV1D-39
• Long gene name
  immunoglobulin kappa variable 1D-39
• GenBank acession
  • X59312
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28893
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000159962

Gene info

• Identity
  HGNC:5651
• Gene
  IGHV4-39
• Long gene name
  immunoglobulin heavy variable 4-39
• GenBank acession
  • L10094
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28394
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152067

Gene info

• Identity
  HGNC:12925
• Gene
  ZNF140
• Long gene name
  zinc finger protein 140
• Synonyms gene name
  • zinc finger protein 140 (clone pHZ-39)
• Synonyms
  • pHZ-39
• GenBank acession
  • U09368
• Locus
  12q24.33
• Discovery year
  1993-02-11
• Entrez gene record
  7699
• Pubmed identfication
  • 7557990
• RefSeq identity
  • NM_003440
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000167942

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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