CD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: Alexa Fluor 647 conj. Clone: 10/78; antibody-Rat, Triple Color Reagent Cocktail; flow
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Catalog numberYSRTTC019
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PricePlease ask
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Sizevial
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Alternative name 1CD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: Alexa Fluor 647 conj. Clone: 10/78; anti-Rat, Triple Color Reagent Cocktail; flow
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Alternative name 2CD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: Alexa Fluor 647 conj. Clone: 10/78; antibody-Rat, Triple Color Reagent Cocktail; flow
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Alternative name 3CD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: Alexa Fluor 647 conj. Clone: 10/78; antibody-Rattus norvegicustus norvegicus, Triple Color Reagent Cocktail; flow
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Alternative name 4anti-CD3: Clone: 1F4/ CD45RA: Clone: OX-33/ CD161: Alexa Fluor 647 Clone: 10/78 Triple Color Reagent Cocktail flow
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Alternative name 5CD3: Clone: 1F4/ CD45RA: Clone: OX-33/ CD161: Alexa Fluor 647 Clone: 10/78 Triple Color Reagent Cocktail flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. accurate-monoclonals made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the CD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: conj. Clone: 10/78; antibody- , Triple Color Reagent Cocktail; exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAlexa Fluor, ALEXA FLUOR 647, Anti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolRPE, HCRT, CD200, TNFSF4, KLRB1, SNORD115-33, IGKV1D-33, IGLV2-33, IGHV3-33, IGHVII-33-1
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Short nameCD3: FITC conj. Clone: 1F4/ CD45RA: RPE conj. Clone: OX-33/ CD161: conj. Clone: 10/78; antibody- , Triple Color Reagent Cocktail;
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TechniqueAntibody, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein
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HostRat
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LabelFITC
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SpeciesRat, Rats
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Alternative nameCD3: fluorecein conj. clonality: 1F4/ CD45RA: RPE conj. clonality: OX-33/ CD161: Alexa Fluor 647 conj. clonality: 10/78; (antibody to-)-Rat, Triple Color Reagent Cocktail; flow
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehypocretin neuropeptide precursor
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Synonyms gene name
- hypocretin (orexin) neuropeptide precursor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD200 molecule
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Synonyms gene
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Synonyms gene name
- antigen identified by monoclonal antibody MRC OX-2
- CD200 antigen
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTNF superfamily member 4
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Synonyms gene
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Synonyms gene name
- tax-transcriptionally activated glycoprotein 1, 34kD
- tumor necrosis factor (ligand) superfamily, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-15
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekiller cell lectin like receptor B1
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Synonyms gene
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Synonyms gene name
- killer cell lectin-like receptor subfamily B, member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-01-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Killer cell lectin like receptors
- C-type lectin domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-33
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-33
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-33 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 2-33
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-33
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-33-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-33-1
- immunoglobulin heavy variable (II)-33-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID