MP qPCR Master Mix (2x) For quantitative multiplex real-time PCR and two-step real-time RT-PCR using sequence-specific probes.
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Catalog number
E0424-02
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Price
Please ask
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Size
2X200 reactions
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Group
PCR, polymerase chain reaction, RT-PCR mixes
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
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Description
Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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Gene target
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Short name
MP qPCR Master (2x) For quantitative multiplex real-time PCR two-step real-time RT-PCR using sequence-specific probes.
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Technique
multiplex, Real-time, RT-PCR, step, PCR, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. steps
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Alternative name
MP Quantitative real-time PCR test kit Master Mix (2x) to measure volumetric multiplex real-time PCR test kit and two-step real-time Reverse transcription PCR test kit using sequence-specific probes.
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Alternative technique
pcrkits, rtpcr, rtpcrkits, steps, dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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