MP qPCR Master Mix (2x), plus ROX Solution For quantitative multiplex real-time PCR and two-step real-time RT-PCR using sequence-specific probes. Contains ROX dye.

• Catalog number
  E0425-01
• Price
  Please ask
• Size
  3X100 reactions

• Group
  PCR, polymerase chain reaction, RT-PCR mixes
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
• Description
  Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.

• Gene target
  qPCR   Master   plus   For   quantitative   two   using   specific   probes   Contains   dye  
• Short name
  MP qPCR Master (2x), plus ROX For quantitative multiplex real-time PCR two-step real-time RT-PCR using sequence-specific probes. Contains ROX dye.
• Technique
  multiplex, Real-time, RT-PCR, step, PCR, Rox, multiplexes, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. rox labelled 1. steps
• Label
  dye
• Alternative name
  MP Quantitative real-time PCR test kit Master Mix (2x), plus ROX solution to measure volumetric multiplex real-time PCR test kit and two-step real-time Reverse transcription PCR test kit using sequence-specific probes. Contains ROX dye.
• Alternative technique
  pcrkits, rtpcr, rtpcrkits, steps, realtime, dna-amplification

MeSH Data

• Name
  Polymerase Chain Reaction
• Concept
  Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
• Tree numbers
  • E05.393.620.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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