Fast Probe qPCR Master Mix (2x), plus ROX Solution For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR using sequence-specific probes. Contains ROX dye.
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Catalog number
E0423-03
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Price
Please ask
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Size
1 000 reactions
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Group
PCR, polymerase chain reaction, RT-PCR mixes
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
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Description
any of various testing devices or substances as (1) a pointed metal tip for making electrical contact with a circuit element being checked (2) a usually small object that is inserted into something so as to test conditions at a given point (3) a device used to penetrate or send back information especially from outer space or a celestial body (4) a device (as an ultrasound generator) or a substance (as radioactively labeled DNA) used to obtain specific information for diagnostic or experimental purposes, Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
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Gene target
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Short name
Fast Probe qPCR Master (2x), plus ROX For fast-cycling quantitative real-time PCR two-step real-time RT-PCR using sequence-specific probes. Contains ROX dye.
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Technique
Real-time, RT-PCR, probe, step, PCR, Rox, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. probes, rox labelled 1. steps
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Label
dye
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Alternative name
Fast Probe Quantitative real-time PCR test kit Master Mix (2x), plus ROX solution to measure fast-cycling volumetric real-time PCR test kit and two-step real-time Reverse transcription PCR test kit using sequence-specific probes. Contains ROX dye.
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Alternative technique
rtpcr, rtpcrkits, probes, steps, realtime, dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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