Recombinant Mouse ENPP7 / NPP-7 Protein (His tag)

• Catalog number
  PKSM040611-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  Alk-SMase;Gm254
• Activity
  NA
• Sequence
  Met 1-Gln 421
• Fusion tag
  C-His
• Accession
  Q3TIW9
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  47 kDa
• AP Mol Mass
  60 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6
• Background
  Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ENPP7   NPP-7   Protein   His   tag  
• Gene symbol
  ENPP7, PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, SEC13, PIRC29
• Short name
  Recombinant Mouse ENPP7 / NPP-7 Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse ENPP7 / NPP-7 Protein (histidine detection labelled)
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:23764
• Gene
  ENPP7
• Long gene name
  ectonucleotide pyrophosphatase/phosphodiesterase 7
• Synonyms
  • alk-SMase
  • NPP7
• Synonyms name
  • alkaline sphingomyelinase
• GenBank acession
  • AY230663
• Locus
  17q25.3
• Discovery year
  2004-04-29
• Entrez gene record
  339221
• Pubmed identfication
  • 12885774
• RefSeq identity
  • NM_178543
• Classification
  • Ectonucleotide pyrophosphatase/phosphodiesterase family
• VEGA ID
  OTTHUMG00000177462

Gene info

• Identity
  HGNC:37538
• Gene
  PIRC34
• Long gene name
  piwi-interacting RNA cluster 34
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313890
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37537
• Gene
  PIRC33
• Long gene name
  piwi-interacting RNA cluster 33
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313854
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37536
• Gene
  PIRC32
• Long gene name
  piwi-interacting RNA cluster 32
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313811
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:32995
• Gene
  SNORD114-7
• Long gene name
  small nucleolar RNA, C/D box 114-7
• Synonyms
  • 14q(II-7)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767583
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003199.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5633
• Gene
  IGHV3OR15-7
• Long gene name
  immunoglobulin heavy variable 3/OR15-7 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3/OR15-7
  • immunoglobulin heavy variable 3/OR15-7 pseudogene
• Synonyms
  • IGHV3/OR15-7
• GenBank acession
  • Z29597
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28318
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000171835

Gene info

• Identity
  HGNC:33026
• Gene
  SNORD115-7
• Long gene name
  small nucleolar RNA, C/D box 115-7
• Synonyms
  • HBII-52-7
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033444
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003299.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:34284
• Gene
  RNU6-7
• Long gene name
  RNA, U6 small nuclear 7
• Synonyms
  • U6-7
• Locus
  14q12
• Discovery year
  2008-06-10
• Entrez gene record
  101954275
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_104084
• Classification
  • Small nuclear RNAs

Gene info

• Identity
  HGNC:10697
• Gene
  SEC13
• Long gene name
  SEC13 homolog, nuclear pore and COPII coat complex component
• Synonyms gene
  • D3S1231E
  • SEC13L1
• Synonyms gene name
  • SEC13 (S. cerevisiae)-like 1
  • SEC13-like 1 (S. cerevisiae)
  • SEC13 homolog (S. cerevisiae)
• Synonyms
  • SEC13R
  • npp-20
• Locus
  3p25.3
• Discovery year
  1994-12-15
• Entrez gene record
  6396
• Pubmed identfication
  • 7987303
  • 15146057
  • 18692470
• RefSeq identity
  • NM_001136026
• Classification
  • GATOR2 subcomplex
  • COPII coat complex
  • WD repeat domain containing
  • Nucleoporins
• VEGA ID
  OTTHUMG00000128671

Gene info

• Identity
  HGNC:37533
• Gene
  PIRC29
• Long gene name
  piwi-interacting RNA cluster 29
• Locus
  7
• Discovery year
  2009-11-05
• Entrez gene record
  100313927
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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