IL15 protein His tag

Category

Proteins
Antibody Subtype

Conjugated Proteins
Area of research

Cytokines & Growth Factors
Reactivity

Mouse
Residues

49-162 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Conjugate Tag

His tag
Type of protein

Recombinant
Expression System

Escherichia coli
Grade Purity

Grater than 90% pure
Concentration

0.5 mg/ml (lot specific)
Form Buffer

Provided as a liquid in PBS, pH7.4, containing 10% glycerol.
Storage

Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

SDS-PAGE
Bioactivity

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml.
Assay Information

Cell line: CTLL2 (mouse cytotoxic Tcell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay Medium: 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay
Additional Information

IL15 protein has been used in SDS PAGE , and may be suitable for use in other assays, to be determined by the end user.
URL
More info
Conjugation

histidine

Gene target

IL15 protein His tag
Gene symbol

IL15
Short name

IL15 protein His tag
Technique

Tags
Label

His
Alternative name

Purified recombinant Mouse IL15 protein
Alternative to gene target

interleukin 15, IL-15, IL15 and IDBG-39028 and ENSG00000164136 and 3600, protein binding, nuclei, Il15 and IDBG-171492 and ENSMUSG00000031712 and 16168, IL15 and IDBG-629577 and ENSBTAG00000018200 and 281248