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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Cytokines & Growth Factors
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Reactivity
Mouse
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Residues
21-169 amino acids: MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 90% pure
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Concentration
0.5 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in PBS, pH7.4, containing 20% glycerol, and 0.1M NaCl.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
The ED50 for this effect is < 2.5 ng/ml. Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml.
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Assay Information
Cell line: CTLL2 (mouse cytotoxic Tcell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay Medium - 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay
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Additional Information
IL2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugation
histidine
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Gene
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. Rec. E. coli interleukin-2 for T cell culture or antibody production is supplied by GENTAUR. Free samples on request.
