Recombinant Human nemo-like kinase / NLK Protein (His & GST tag) 50µg

• Catalog number
  PKSH030360-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  LAK1;NLK
• Activity
  The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
• Sequence
  Val121-Glu527
• Fusion tag
  N-His & GST
• Accession
  Q9UBE8
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞91 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  74.1 kDa
• AP Mol Mass
  73 kDa
• Formulation
  Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Reconstitution
  Please refer to it for detailed information.
• Background
  Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  nemo-like   kinase   NLK   Protein   His   GST   tag   50µg  
• Gene symbol
  NLK, MGST3, GPI
• Short name
  Recombinant nemo-like kinase / NLK Protein (His & GST tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens nemo-like phosphorylation catalyst / nemo-like kinase Protein (histidine & GST detection labelled) 50µg
• Alternative technique
  rec
• Alternative to gene target
  nemo-like kinase, NLK and IDBG-36215 and ENSG00000087095 and 51701, transferase activity, nuclei, Nlk and IDBG-203133 and ENSMUSG00000017376 and 18099, NLK and IDBG-631542 and ENSBTAG00000014825 and 507204

Gene info

• Identity
  HGNC:29858
• Gene
  NLK
• Long gene name
  nemo like kinase
• Synonyms gene name
  • nemo-like kinase
• GenBank acession
  • AF197898
• Locus
  17q11.2
• Discovery year
  2004-02-19
• Entrez gene record
  51701
• Pubmed identfication
  • 9448268
  • 10863097
• RefSeq identity
  • NM_016231
• VEGA ID
  OTTHUMG00000132451

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:4458
• Gene
  GPI
• Long gene name
  glucose-6-phosphate isomerase
• Synonyms gene name
  • glucose phosphate isomerase
• Synonyms
  • AMF
  • NLK
• GenBank acession
  • M61214
• Locus
  19q13.11
• Discovery year
  2001-06-22
• Entrez gene record
  2821
• Pubmed identfication
  • 2387591
  • 8575767
• VEGA ID
  OTTHUMG00000182087
• Locus Specific Databases
  • LRG_1178

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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