Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag) 50µg
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Catalog number
PKSH030343-50µg
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Price
Please ask
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Size
50µg
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Synonym
DAPK
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Activity
The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
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Sequence
Met 1-Leu 363
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Fusion tag
N-His & GST
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Accession
P53355-1
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Expressed Host
Baculovirus-Insect Cells
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Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity
＞80 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
69.4 kDa
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AP Mol Mass
64 kDa
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Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
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Reconstitution
Please refer to it for detailed information.
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Background
Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
DLGAP1, DAP, DAPK1-IT1, DAPK1, MIR363, MGST3, DAP-DT, DNPEP, IAPP, GNPAT
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Short name
Recombinant DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag) 50µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens death-associated protein kinase 1 / death-associated protein phosphorylation catalyst 1 Protein (aa 1-363, histidine & GST detection labelled) 50µg
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Alternative technique
rec
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Alternative to gene target
death-associated protein, DAP and IDBG-12509 and ENSG00000112977 and 1611, death domain binding, multiple, Dap and IDBG-132573 and ENSMUSG00000039168 and 223453, DAP and IDBG-631574 and ENSBTAG00000006346 and 616066
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Gene info
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Identity
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Gene
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Long gene name
DLG associated protein 1
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Synonyms gene name
- discs, large (Drosophila) homolog-associated protein 1
- discs large homolog associated protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-02-23
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
death associated protein
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GenBank acession
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Locus
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Discovery year
1995-04-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 363
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2005-06-30
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
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Identity
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Gene
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Long gene name
DAP divergent transcript
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Locus
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Discovery year
2020-08-21
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Entrez gene record
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Classification
Gene info
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Identity
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Gene
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Long gene name
aspartyl aminopeptidase
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Synonyms
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Locus
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Discovery year
1998-09-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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