Recombinant Human CD32b / FCGR2B Protein (His & AVI tag)

• Catalog number
  PKSH031725-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  CD32;CD32B;Fc gamma RIIB;FCGR2
• Activity
  Immobilized human CD32b-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.20-0.48 μg/ml.
• Sequence
  Ala 46-Ile 224
• Fusion tag
  C-His & AVI
• Accession
  NP_001002274.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  24 kDa
• AP Mol Mass
  30-35 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD32b   FCGR2B   Protein   His   AVI   tag  
• Gene symbol
  FCGR2B
• Short name
  Recombinant CD32b / FCGR2B Protein (His & AVI tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens CD32b / fragment c fragment on Immunoglobulin G, low affinity IIb, receptor (CD32) Protein (histidine & AVI detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  Fc fragment of IgG, low affinity IIb, receptor (CD32), CD32 and CD32B and FCG2 and FCGR2 and IGFR2, FCGR2B and IDBG-104308 and ENSG00000072694 and 2213, IgG binding, Plasma membranes

Gene info

• Identity
  HGNC:3618
• Gene
  FCGR2B
• Long gene name
  Fc gamma receptor IIb
• Synonyms gene
  • FCG2
  • FCGR2
• Synonyms gene name
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG receptor IIb
• Synonyms
  • CD32
  • CD32B
  • FcgammaRIIb
  • FcGRIIB
• GenBank acession
  • BC031992
• Locus
  1q23.3
• Discovery year
  1991-08-21
• Entrez gene record
  2213
• Pubmed identfication
  • 2139735
  • 19309690
• RefSeq identity
  • NM_004001
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000034470

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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