Rat CASP1(Caspase 1) EIA Kit
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Catalog number
E-EL-R0371-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
ICE, IL1BC, P45, Interleukin 1 Beta Converting Enzyme, Apoptosis-Related Cysteine Peptidase
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP1. You can calculate the concentration of Rat CASP1 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
37.5pg/mL
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Detection Range
62.5~4000pg/mL
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Target Name
CASP1
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UNIProt ID
P43527
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat CASP1(Caspase 1) EIA Kit
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Type
Sandwich
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Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
CASP1
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Short name
CASP1(Caspase 1) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat caspase 1, apoptosis-related cysteine peptidase(caspase 1) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
caspase 1
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Synonyms gene
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Synonyms gene name
- caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase)
- caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
- caspase 1, apoptosis-related cysteine peptidase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-05-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
Product images
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